Chii ropa serum

Serum chinhu chimwe chakafanana nawo plasma, isina chimiro zvinhu uye fibrin. It achiumbwa somugumisiro zvimwe nemakemikari. Kuwana serum nzira mbiri: neutralizing fibrinogen karusiyamu ions uye somugumisiro yepanyama kweropa.

Muitiro rokuzvigadzira rayo rinonzi "defibrinirovanie". Yemazuva ano, zviri sezvinotevera: midziyo unganidzwa ropa nomuzvarirwo collapses, akatendeuka kuva hwakasimba fibrin clot. Last rinoburitsa masero eropa uye mazhinji akamira zvishoma nezvishoma akakudzvinya kubva pachake yero zvokunwa. Iri ndiro ropa serum.

serum ruvara nokuda kuvapo mairi imwe mari bilirubin. Zvibereko zvayo rinoratidza kuvapo ruvara muviri zvirwere. Inowanzotorwa serum segirazi. Asi pashure kudya zvishoma ramakore, izvo akwanise nokuda admixture mafuta mumate. The pose Makakatanwa pakati ropa serum dererei zvikuru kudarika mvura.

The zvinoitika Kuwanda serum mapuroteni riri pakati matanhatu navasere muzana. In kwayo sezvamunoita rine kunyanya sealbumin (4.5-6.5%) uye globulin, (1.9-2.2%). Changing kukura izvi puroteni yeimwe dzavo quantitative nokufamba vari mukuru kunotoda kukosha. Zvisinei, nyaya iyi haisi aizvinzwisisa.

Refraction serum anoramba risina kuchinjwa nokupesvedzera vasiri zvinhu, zvakadai yokuwana hydrotherapy kana nguva zvokudya. Asi kwenguva refu kutsanya kungaita kuti vasanyanye padanho mapuroteni ari serum. Mukupesana, kuti tsandanyama basa iri rinenge hapana sei ayo refraction.

Kuwa uwandu mapuroteni muropa serum iri kuchengetwa chakasimba zvinotapukira. Padanho mapuroteni yeimwe oga ikadzoka dzose munguva pakupora nguva. An kunze ndiko tibhii, sezvamakaropafadzwa yaipedzerwa mapuroteni, kunyanya globulin, inowedzerawo yekudyidzana.

Panyaya application, kupfuura inowanzoshandiswa ropa serum munguva nokukanganisika ropa kuongorora inodzidza kuti zvirwere, kuongorora kubudirira kubaya uye kuziva boka.

Pari, akasiyana nzira mbiri zvinoshandiswa okurapa, rimwe ropa boka mureza Sera. Kuti kusakanganisa, shandisa chete kushingaira serum dzakakwirira titer. Research chiri kuitwa mumba umo tembiricha haafaniri kupfuudza 25 Celsius. Migumisiro inofanira dzinoongororwa kwete kwokutanga pane pashure maminitsi 5 kubva pakutanga kudzidza.

Equipment nzira iyi ndiyo sezvinotevera. Pakutanga, zvinokosha kuti tizive kuti titer pamusoro serum dilution izvo hazvifaniri kuva zvishoma pane chimwe muna matatu. Nokuti chinangwa ichi, mumwe nomumwe sepombi rakundwa kumativi samadonhwe maviri makuru, ayo anechekuita munhu sandarara pamusoro. Zvadaro, mumwe nomumwe samadonhwe izvi vachiwedzerwa nemaune inogruppnye erythrocytes uye pakakodzera pamwe serum. Mushure memaminitsi mashanu, izvozvo kwakatemwa okupedzisira donhwe, kwakaitika agglutination. Ndicho chikuru dilution. Izvi anonzi "serum titer gemagtlyutiniruyuschey".

Next, uri girazi ndiro kana achishandisa pipettes rinoshandisirwa chikuru donhwe yemabhii serum, uye ipapo girazi shamhu hwakabatana ropa rayo madonhwe. Mushure maminitsi mashanu, mumwe nomumwe musanganiswa Wakawedzerwa donhwe nomumwe kuderera yesaline, uye ipapo kuyera zvabuda. Izvi ndizvo zvose kuti inyaya muitiro dzokuziva mapoka ropa pamureza Sera.

Achipfupikisa ose pamusoro, zvinofanira kucherechedzwa kuti nhasi ari serum - haisi chete zvakakodzera reagent, asiwo huru vanoshingaira wemafuta zvinodhaka zhinji yaishandiswa kurapa vakawanda zvirwere.